How to use this site

Two experiments, one shared workflow. Follow the steps below — tools are linked to each stage.

🔬
Before the
experiment
Understand the design & variables
Both
🌿
Chlorophyll
absorption
Choose which light colours to test
Photosynthesis
🟢
Inspect
spheres
Check uniformity before loading
Both
🧪
Run the
experiment
Incubate & collect buffer samples
Both
📊
Plate reader
scan
Measure absorbance; export CSV
Both
🌱
Photosynthesis
Analyser
Upload CSV + calibration; get ΔpH
Photosynthesis
🫁
Respiration
Analyser
Upload CSV + calibration; get ΔpH
Respiration
📋
Review
assumptions
Evaluate errors & validity
Both
Tools on this site
Experimental Design Walkthrough
Explains every design choice and controlled variable in both experiments, with links to the Assumptions Catalogue.
Use before & after your experiment
Chlorophyll Light Absorption
Interactive chart overlaying chlorophyll a & b absorption spectra with your LED light box spectra.
Use before the photosynthesis experiment
Sphere Uniformity Checker
Enter sphere measurements to quantify size variation and identify outliers before loading vials.
Use at Step 2 of each experiment
Plate Reader Simulator
3D simulation of the 96-well plate reader. Load pH standards and practice reading absorbance values.
Use before your first plate reader session
Photosynthesis Analyser
Upload your plate reader CSV and calibration file. Assigns wells, averages replicates, and outputs ΔpH per light condition.
Use after the plate reader scan (595 nm)
Respiration Analyser
Upload your plate reader CSV and calibration file. Assigns wells, averages replicates, and outputs ΔpH per temperature condition.
Use after the plate reader scan (450 nm)
Calibration Tool
Build and export calibration curves as JSON files for use in the Analyser tools. For facilitator use.
Prepared by facilitator before sessions
Assumptions Catalogue
18 experimental assumptions, each with residual uncertainty, error type, and VCE discussion prompts. Expandable cards.
Use after analysing your results